Buses will replace Craigieburn Line trains between Essendon and Broadmeadows from 9pm, Friday, July 22 to the last service on Sunday, July 24.
Buses will then replace all trains between Essendon and Craigieburn from the first to the last service on Monday, July 25, the Department of Transport said.
Passengers are asked to allow up to 40 minutes extra for their journey.
