The Altona-bound exit from the M80 Ring Road to the Hume Freeway and the Edgars Road Altona-bound entry ramp to the ring road will close on Monday night.
The Department of Transport said the Altona-bound exit to the Hume Freeway would be closed from 10pm to 5am as part of the M80 upgrade.
A detour will be in place via Sydney Road and Cooper Street.
The Edgars Road Altona-bound entry ramp will be closed 9pm to 5am.
Motorists can detour via Mahoneys Road and enter from Sydney Road. To reach the Hume Freeway, use Edgars Road and Cooper Street.